[notmattlythgoe]

But I dont think I have assigned same channels to two or more than two DIO s!
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Check to make sure that you have initialized all of the DIOs that you are using. We found out recently that if you forget to initialize something in the Begin.vi it will try to use the default values for it. This could be trying to create a default one on your Slot 4 DIO 1 channel. Also while it is trying to figure out the default it will cause a delay that triggers the watchdog problems.